Capture and Release of Viruses through Amino-Functionalized Silica Particles

Description:

Background:
 
A new virus capture/release strategy for the concentration of viral particles in water is reported. The method is an improvement to traditional approaches that rely exclusively upon electrostatic attractions between a charged substrate and charged viral particles, which can only be reversed under extremely acidic or alkaline conditions to effect surface charge reversal and subsequent release of captured viruses.
 
Technology:
 
The nanobead virus capture and release technology is useful for concentrating viruses in a complex matrix, as opposed to prefiltering. 

The patented method utilizes negatively charged silica beads functionalized with amino-groups using defined length spacer molecules to yield particles with a surface density that is optimized for efficient virus capture. Following capture, viruses can be released using soluble proteins or amino acid-based alkaline eluents.

 

Beads are functionalized with 3-(ethylenediamino)propy surfac (EPS), 3-(diethyenetriamino)propy surface (DPS), 3-aminopropyl surface or Lupamin surface treatments, all which have a measureable affinity to viruses. 

 

 

 

 

Advantages:

 

Virus capture efficiencies that exceed 95%.

Capture viruses in a wide range of concentrations and solution pH ranges.

Effective in large volume, high flow through applications.

 

Patent Status: US Patent issued

 
Link to the technology announcement:
 
http://ott.testtechnologypublisher.com/files/sites/virus-capture.pdf

Patent Information:
Category(s):
Microfluidic Devices
For Information, Contact:
Tim Joyce
Technology Commercialization Manager
University of Notre Dame
(574) 631-3029
tjoyce2@nd.edu
Inventors:
Hsueh-Chia Chang
Zilin Chen
Keywords:
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