Affinity-based Precipitation for Non-chromatographic Purification of Bivalently Active Antibodies

Description:

An affinity-based precipitation method for the rapid and nonchromatographic purification of bivalently active monoclonal antibodies combines the selectivity of affinity chromatography with the simplicity of salt-induced precipitation.  The process demonstrates the abililty to purify antibodies from typical contaminants including CHO cell conditioned media, ascites fluid, DNA, and other antibodies with yields >85% and with >95% purity.  Purified antibodies display native binding levels to cell lines expressing the target proteins. Selectivity of the affinity-based precipitation method for bivalent activity can be established by purifying solutions containing both active and chemically denatured antibodies. Prior to purification, solutions can displayed 20−76% reduction in binding activity, where after purification, native binding activity is restored with the purified product containing only bivalently active antibodies.

 

Key Features and Benefits:

1.       Reduced purification costs

2.       Increased purified product quality

3.       Affinity-based precipitation method does not adversely affect the antibodies

4.       Rapid and straightforward process for purification of antibodies

 

Patent Information:
For Information, Contact:
Gaylene Anderson
Sr. Innovations Officer
University of Notre Dame
574-631-9692
andersg4@ccf.org
Inventors:
Zihni (Basar) Bilgicer
Michael Handlogten
Jared Stefanick
Nathan Alves
Tanyel Kiziltepe-Bilgicer
Keywords:
© 2017. All Rights Reserved. Powered by Inteum