System and Method for Image Resolution in Multiphoton Microscopy


Ever wonder why images of living cells and viruses are fuzzy? The Raleigh, or Abbe, limit establishes that one cannot resolve two objects within a half a wavelength of the impinging signal. This makes it particularly difficult to image living cells. That is, until a new discovery was made.


In 2014, the Nobel Prize in Chemistry was awarded to Erik Betzig, Stefan W. Hell and W. E. Moerner for the development of super-resolution fluorescence microscopy. With knowledge of the nature of fluorescence diffusion and employing multiple measurements, it was found that one could actually improve image resolution far past the Raleigh limit using a multi-photon microscope.


Now, researchers at the University of Notre Dame have made yet another significant leap in resolution. By taking advantage of information in distortion created by over-saturation, it is possible to both improve the image by an order of magnitude (in the range of 10-100nm) and, for the first time, quantitatively determine concentration. Now, a multi-photon microscope, known for its safe irradiation and deep 3D imaging capabilities, can image an isolated in-vitro living cell with immense clarity. Now, the same microscope can be used to accurately measure concentration, as well.


Patent Information:
Life sciences
For Information, Contact:
Richard Cox
Director, Licensing & Business Development
University of Notre Dame
(574) 631-5158
Scott Howard
Genevieve Vigil
Yide Zhang
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